The Frances A. Johnston and Charlotte M. Young Human Metabolic Research Unit (HMRU) is a 3,830 square-foot unit with specialized laboratories and facilities that support comprehensive nutrition and metabolism research on human subjects.
The HMRU is used to support research on human metabolism, dietetics, reproductive physiology and clinical chemistry, as well as studies on human performance and assessment of body composition and bone mass.
It is a shared resource for nutrition research and education in the DNS and is administered through the office of the DNS director.
All research conducted in the HMRU is approved by Cornell University's Institutional Review Board (IRB) and subject to various HMRU policies and standard operating procedures. Any faculty member acting as the principal investigator or the supervising faculty member of any project being conducted in the HMRU is responsible for all aspects of the scientific conduct of the project.
This laboratory supports research on nutritional and biochemical biomarkers. The research focus is primarily on human nutrition,but the laboratory also is used to conduct numerous assays on samples from other species.
The laboratory routinely runs assays using a Siemens Immulite 2000 automated immunoassay system and a Siemens Dimension Xpand Chemistry Analyzer. The laboratory also is equipped with a Biotek Synergy 2 microplate reader, a Biotek Precision XS Robotic Systemanda Biotek ELx50 magnetic microplate washer to do more specialized immunoassays using commercially available kits.
For hematology analysis, the laboratory is equipped with a Beckman-Coulter AcT Diff 2 coulter counter. The laboratory also has a Parr Oxygen Combustion Bomb Calorimeter for food and tissue calorimetric studies.
For sample processing, the laboratory has numerous centrifuges, a biosafety cabinet, and a Savant DNS120 Speedvac. Short-term and long-term storage of samples is available in three 80°C ultra-low freezers.
The laboratory also uses the Luminex Magpix Multiplex System and the Quantimetrix Lipoprint LDL and HDL subfraction system.
The Magpix is a multiplex immunoassay system that uses magnetic bead technology, with LED optics and charge-couple base device (CCD) camera to illuminate and detect differentially fluorescent dyed beads. The technology allows for highly sensitive quantification of up to 50 biomarkers at a time on one 96 well plate. The technology requires less time, less sample volume, and less cost compared with conventional ELISA systems. To findout more about this technology please visit: http://www.luminexcorp.com/Products/Instruments/MAGPIX/index.htm
A list of multiplex panels for the Magpix that are available along with specifications are listed on this spreadsheet (Multiplex_Analytes_Summary.xls)
The Lipoprint system uses high resolution gel electrophoresis to separate and quantitate both High Density Lipoprotein (HDL) and Low Density Lipoprotein (LDL) subfractions, as well as Intermediate Density Lipoprotein (IDL) fractions and Very Low Density Lipoprotein (VLDL). It reduces sample volume; cost and time compared to density ultracentrifugation and nuclear magnetic resonance methodologies. The Lipoprint LDL method is the only subfraction methodology that has FDA approval for clinical testing. http://quantimetrix.com/lipoprint/
For question or additional information contact:
The CSCU is a professional service group that helps scholars optimally employ statistical methods in their research.
Unit staff members provide statistical expertise to the entire Cornell community through consulting services, instruction, workshops, training, instructional materials and contract services.
The consulting services can help you design experiments and surveys, offer statistical support for your research and grant proposals, select the most appropriate statistical analyses for your work, interpret your findings, prepare your presentations, as well as other services.
CSCU also offers workshops every semester on statistical topics commonly used by researchers. The workshops are offered free of charge to members of the Cornell community.
Through a periodic newsletter, bulletin, handouts and reference library, the CSCU provides helpful instructional materials to assist you in your research.
For a fee, the unit also will do the statistical analysis for you. The service is available to the Cornell community and to external clients.