Human Nutritional Chemistry Service Laboratory
The Human Nutritional Chemistry Service Laboratory (HNCSL), located on the 2nd floor of the new Human Ecology Building (HEB), provides research support in the area of nutritional and biochemical biomarkers. The focus is primarily on human nutrition; however, the laboratory is capable of doing numerous assays on samples from other species. The laboratory routinely runs assays using a Siemens Immulite 2000 automated immunoassay system and a Siemens Dimension Xpand Chemistry Analyzer. The laboratory also has a Biotek Synergy 2 microplate reader, a Biotek Precision XS Robotic System, a Biotek ELx50 magnetic microplate washer to do more specialized immunoassays using commercially available kits. For hematology analysis, the laboratory has a Beckman-Coulter AcT Diff 2 coulter counter. The laboratory also possesses a Parr Oxygen Combustion Bomb Calorimeter for food and tissue calorimetric studies. For sample processing, the laboratory has numerous centrifuges, a biosafety cabinet, and a Savant DNS 120 Speedvac. For a fee, short term and long term storage of samples is available in our three -80°C ultra low freezers. Space is limited to samples acquired in human participant studies. For added security, the freezers are locked and tied into a central alarm system. The freezers are centralized in a card swipe access only room and inventory of samples are organized using FreezerWorks software with barcode capability. The two newest additions to the laboratory are the Luminex Magpix Multiplex System and the Quantimetrix Lipoprint LDL and HDL subfraction system.
The Magpix is a multiplex immunoassay system that uses magnetic bead technology, with LED optics and charge-couple base device (CCD) camera to illuminate and detect differentially fluorescent dyed beads. The technology allows for highly sensitive quantification of up to 50 biomarkers at a time on one 96 well plate. The technology requires less time, less sample volume, and less cost compared to conventional ELISA systems. To find out more about this technology visit: http://www.luminexcorp.com/Products/Instruments/MAGPIX/index.htm
A list of multiplex panels for the Magpix that are available along with specifications are listed on this spreadsheet (Multiplex_Analytes_Summary.xls)
The Lipoprint system uses high resolution gel electrophoresis to separate and quantitate both High Density Lipoprotein (HDL) and Low Density Lipoprotein (LDL) subfractions, as well as Intermediate Density Lipoprotein (IDL) fractions and Very Low Density Lipoprotein (VLDL). It reduces sample volume; cost and time compared to density ultracentrifugation and nuclear magnetic resonance methodologies. The Lipoprint LDL method is the only subfraction methodology that has FDA approval for clinical testing. http://quantimetrix.com/lipoprint/