Shu-Bing Qian

 

Shu-Bing Qian

Associate Professor
301 [Office] & 303 [Lab] Biotech Bldg
 
Phone: (607) 254-3397
Email: sq38@cornell.edu
View Cornell University Contact Info
Curriculum Vitae
 
Biographical Statement:

Dr. Shu-Bing Qian received his MSc and PhD degrees in Molecular Biology & Biochemistry with honors in 1997 and 2000, respectively, from Shanghai Jiaotong University Medical School (formerly Shanghai Second Medical University). He then conducted two postdoctoral fellowships at the National Institutes of Health (Bethesda, MD) and University of North Carolina (Chapel Hill, NC) in the field of Biochemistry. Dr. Qian became an Assistant Professor in the Division of Nutritional Sciences at Cornell University in July 2008.  In 2009, he received Young Investigator Award from Ellison Medical Foundation, and NIH Director's New Innovator Award. In 2010, Dr. Qian received DOD Development Award. In 2013, Dr. Qian received Peter Reeds Young Investigator Award.

Most of the research work in Dr. Qian's laboratory is broadly interdisciplinary, with a primary emphasis on the nutrient signaling pathway, stress response, protein synthesis, and their implications in human diseases. Using biochemical, genetic, and cell biological approach, the Qian laboratory investigates nutritional and genetic determinants of adaptive stress response in growth and aging. Specific research interests include chaperone network and ubiquitin/proteasome system, nutrient sensing pathway mTOR (the mammalian target of rapamycin), and translational regulation of gene expression. Elucidation of regulatory mechanisms may help develop therapeutic strategies for human diseases, such as diabetes, cancer, and neurodegenerative disorders.

 
Teaching and Advising Statement:

Teaching is an exciting, enriching, and an integral component of an academic career, and I am firmly committed to excellence in teaching.  My primary goal is to instill interest and to expose students to the course topics, which in turn enables me to achieve a better understanding of the material taught, which then provides all parties an opportunity to learn new material.  Overall, I enjoy teaching and respect this responsibility as a core value and of critical importance to society.

 
Current Professional Activities:

Graduate Field Membership: Nutrition; Genetics & Development; Biochemistry, Molecular & Cellular Biology
Faculty Member: Center for Vertebrate Genomics
Faculty Member: Chemical Biology Interface (CBI) Program
Faculty Member: Leadership Program for Veterinary Students

 
Current Research Activities:

How is mRNA translation controlled by nutrient signaling?  How does protein folding and degradation occur during protein synthesis?  How do cells get rid of misfolded proteins?  These are a few of the problems we would like to understand.  Elucidation of the molecular mechanisms underlying protein quality and quantity control will ultimately define new therapeutic strategies to human diseases such as cancer, diabetes, and neurodegenerative disorders.  

 

Specifically, we use biochemistry, cell biological, and genetic approaches to study translational control of gene expression and protein triage (folding, degradation, and aggregation) using mammalian system.  We established high resolutionribosomal profiling analysis to monitor mRNA translation, which allows us to investigate ribosome dynamics as well as co-translational events. In addition, we are applying genome-wide high-content RNAi screen to dissect protein triage decision.  By focusing on chaperone network and the translation machinery, we are dedicated to elucidate fundamental principles of protein homeostasis.

 
Education:

PostDoc, 2004 ~ 2006  University of North Carolina, Chapel Hill, NC
PostDoc, 2000 ~ 2004  National Institutes of Health, Bethesda, MD
Ph.D., 2000    Shanghai Jiaotong University Medical School, Biochemistry
M.Sc., 1997    Shanghai Jiaotong University Medical School, Biochemistry

 
Courses Taught:

BIOG4990 - Independent Research in Biology II
NS4010 - Empirical Research
NS3200 - Human Biochemistry
NS7030 - Seminar in Nutritional Sciences

 
Related Websites:

Lab: http://qian.human.cornell.edu/

 
Administrative Responsibilities:

Member of DNS Curriculum Committee

 
Selected Publications:

Wan J and Qian SB.  TISdb: a database for alternative translation initiation in mammalian cells. Nucleic Acids Res 2014; 42(1):D845-50. PMID: 24203712

 

Liu B and Qian SB.  Translational reprogrammin in cellular stress response. WIREs RNA 2013 (in press). PMID: 24375939

 

Sherman MY and Qian SB.  Less is more: Improving proteostasis by translation slow-down. Trends Biochem Sci 2013; 38(12):585-91. PMID: 24126073

 

Conn CS and Qian SBmTORC1 in protein homeostasis: increase in protein quantity at the expense of quality. Sci Signal 2013; 6(271):ra24. PMID: 23592839

 

Liu B, Han Y, and Qian SB.  Co-translational response to proteotoxic stress by elongation pausing of ribosomes. Mol Cell 2013; 49(3):453-463. PMID: 23290916

 

Liu B, Conn CS, and Qian SB.  Viewing folding of nascent polypeptide chains from ribosomes. Expert Rev Proteomics 2012; 9(6):579-81. PMID: 23256666

 

Stern-Ginossar N, Weisburd B, Michalski A, Le VT, Hein MY, Huang SX, Ma M, Shen B, Qian SB, Hengel H, Mann M, Ingolia NT, Weissman JS. Decoding human cytomegalovirus. Science 2012; 338(6110):1088-93.  PMID: 23180859

 

Lee S, Liu B, Lee S, Huang SX, Shen B, and Qian SB. Global mapping of translation initiation sites in mammalian cells at single-nucleotide resolution. Proc Natl Acad Sci USA. 2012; 109(37):E2424-32. PMID: 22927429

 

Han Y, David A, Liu B, Magadán JG, Bennink JR, Yewdell JW, and Qian SB. Monitoring co-translational protein folding in mammalian cells at codon resolution. Proc Natl Acad Sci USA. 2012; 109(31):12467-72. PMID: 22802618

 

Park WJ, Kothapalli KS, Reardon HT, Lawrence P, Qian SB, Brenna JT. A novel FADS1 isoform potentiates FADS2-mediated production of eicosanoid precursor fatty acids. J Lipid Res 2012; 53(8):1502-12. PMID: 22619218

 

Liu B, and Qian SB. Translational regulation in nutrigenomics. Adv Nutr 2011; 2(6):511-9

 

Zhang X, and Qian SB. Chaperone-mediated hierarchical control in targeting misfolded proteins to aggresome. Mol Biol Cell 2011; 22(18):3277-88

 

Conn CS and Qian SB.  mTOR signaling in protein homeostasis: less is more? Cell Cycle 2011; 10(12):1940-7

 

Sun J, Conn CS, Han Y, Yeung V, and Qian SB. PI3K-mTORC1 attenuates stress response by inhibiting cap-independent Hsp70 mRNA translation.  J Biol Chem 2011; 286(8):6791-800

 

Qian SB, Zhang X, Sun J, Bennink JR, Yewdell JW, Patterson C. mTORC1 links protein quality and quantity control by sensing chaperone availability.  J Biol Chem 2010; 285(35):27385-95 (co-correspondence author)

 

Qian SB, Waldren L, Choudhary N, Klevit RE, Chazin WJ, Patterson C. Engineering a ubiquitin ligase reveals conformational flexibility required for ubiquitin transfer. J Biol Chem 2009; 284(39):26797-802 (co-correspondence author)

 

McDonough H, Charles PC, Hilliard EG, Qian SB, Min JN, Portbury AL, Cyr DM, Patterson C. Stress-dependent chip/DAXX interaction suppresses the p53 apoptotic program. J Biol Chem 2009; 284(31): 20649-59

 

Xia T , Dimitropoulou C , Zeng J , Antonova GN , Snead C , Venema RC , Fulton D , Qian SB , Patterson C , Papapetropoulos A , Catravas JD . Chaperone-dependent E3 ligase CHIP ubiquitinates and mediates proteasomal degradation of soluble guanylyl cyclase. Am J Physiol Heart Circ Physiol 2007; 293:H3080-3087

 

Qian SB, McDonough H, Boellmann F, Cyr DM, Patterson C. CHIP-mediated stress recovery by sequential ubiquitination of substrates and Hsp70. Nature 2006; 440: 551-555

 

Qian SB, Reits E, Neefjes J, Deslich JM, Bennink JR, and Yewdell JW. Tight linkage between translation and MHC-class I peptide ligand generation implies specialized antigen processing for defective ribosomal products. J Immunol 2006; 177: 227-233

 

Qian SB, Princiotta MF, Bennink JR, Yewdell JW. Characterization of rapidly degraded polypeptides in mammalian cells reveals a novel layer of nascent protein quality control. J Biol Chem 2006; 281(1):392-400

 

Dai Q, Qian SB, Li HH, McDonough H, Borchers C, Huang D, Takayama S, Younger JM, Ren HY, Cyr DM, Patterson C. Regulation of the cytoplasmic quality control protein degradation pathway by BAG2. J Biol Chem 2005; 280(46):38673-38681

 

Shaffer AL, Shapiro-Shelef M, Iwakoshi NN, Qian SB, Zhao H, Yu X, et al. XBP1 acts downstream of Blimp-1 to regulate ER biogenesis, oeganelle expansion, and protein synthesis during plasma cell differentiation. Immunity 2004; 21(1):81-93

 

Princiotta MF, Finzi D, Qian SB, Gibbs J, Schuchmann S, Buttgereit F, Bennink JR, Yewdell JW. Quantitating protein synthesis, degradation, and endogenous antigen processing. Immunity 2003; 18(3):343-354

 

Qian SB, Ott DE, Schubert U, Bennink JR, Yewdell JW. Fusion proteins with COOH-terminal ubiquitin are stable and maintain dual functionality in vivo. J Biol Chem 2002; 277(41):38818-38826

 

Qian SB, Li Y, Qian GX, and Chen SS. Efficient tumor regression induced by genetically engineered tumor cells secreting interleukin-2 and membrane-expressing allogeneic MHC class I antigen. J Cancer Res Clin Oncol 2001; 127(1): 27-33

 

Qian SB, and Chen SS. Blocked transport of soluble Kb molecules containing connecting peptide segment involved in calnexin association. Int Immunol 2000; 12(10): 1409-1416

 

Xie Q, Liao D, Zhou XQ, Qian SB, Cheng SS. Transduction of primary rat hepatocytes with bicistronic retroviral vector. World J Gastroenterol 2000; 6(5):725-729

 

Qian SB, Qian GX, and Chen SS. Enhanced immunogenecity of human hepatocellular carcinoma cells transduced with human gamma-interferon gene via retroviral vector. Acta Univ Med 2nd Shanghai 1999; 11(2): 90-94

 

Qian SB, and Chen SS. Transduction of human hepatocellular carcinoma cell lines transduced with human gamma-interferon gene via retroviral vector. World J Gastroenter 1998; 4(3): 210-213

 

Qian SB, Zhang TF, and Chen SS. Enhanced expression of HLA class I molecules in human hepatocellular carcinoma cell lines transduced with human gamma-interferon gene. Chin Med J (Eng) 1998; 111(4): 319-322

 
Searchable Keywords:
Protein quality control
Nutrients signaling
Protein translation
Stress response
Growth and aging 

 
The information on this bio page is taken from the CHE Annual Report.